SpletPhysical enrichment of transposon mutants from saturation mutant libraries using the TraDISort approach This is a repository copy of Physical enrichment of transposon mutants from saturation mutant libraries using the TraDISort approach. White Rose Research Online URL for this paper: http://eprints.whiterose.ac.uk/116756/ Version: Accepted Version SpletTransposon-directed insertion-site sequencing (TraDIS) is a high-throughput method that couples transposon mutagenesis with short-fragment DNA sequencing. It is commonly …

Developing a method to tease pathogen genomes apart

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The Essential Genome of Escherichia coli K-12 - PubMed

Splet16. okt. 2009 · "Crucially, our new method allows us to achieve all this in just a single experiment." Using the novel method, which the team have named TraDIS (Transposon Directed Insertion site... Splettradis_essentiality.R - Takes a single tab-delimited file from "tradis plot analyse" to produce calls of gene essentiality. Also produces a number of diagnostic plots. tradis_comparison.R - Takes tab files to compare two growth conditions using edgeR. This analysis requires experimental replicates. Splet21. jan. 2016 · We previously developed a method for this purpose, called Tra nsposon D irected I nsertion S equencing (TraDIS; (Langridge et al., 2009). TraDIS uses fragmentation of genomic DNA followed by specific PCR amplification of transposon-containing fragments to selectively enrich for transposon-flanking sequences, and can be adapted … arnautu keramik