WebFeb 17, 2016 · Silencing PPP4R2 in human cell lines, or introduction of a PPP4R2 mutant unable to interact with PPP4C, altered the kinetics and pattern of RPA2 phosphorylation. Depletion of PPP4R2 impeded homologous recombination via inefficient loading of the essential factor RAD51 (179617), causing an extended G2-M checkpoint and … WebSep 2, 2008 · RPA2 phosphorylation follows a preferred pathway so that initial modification by a kinase (e.g., cyclin A-Cdk2) stimulates the subsequent modification by another …
RPA-coated single-stranded DNA as a platform for post
WebDNA-PK also phosphorylates RPA32 in response to replication stress, and we demonstrate that cells with DNA-PK defects, or lacking RPA32 Ser4/Ser8 targeted by DNA-PK, confer … WebRPA32 S4/S8 phosphorylation is a mark of transient Ku association with seDSBs.: (a) Immunoblotting of extracts from U2OS cells pre-treated with the replication inhibitor … hjjjuk
DNA-PK-Dependent RPA2 Hyperphosphorylation Facilitates …
WebNov 23, 2015 · Decreased RPA2 and H2AX phosphorylation might directly account for impaired fork progression at such UV-induced lesions, although how TRAIP promotes H2AX and RPA phosphorylation remains to be … WebPrexasertib Mesylate Hydrate (8-250 nM; pre-treated for 15 minutes) inhibits CHK1 autophosphorylation (S296) and CHK2 autophosphorylation (S516) in HT-29 cells [1]. Prexasertib Mesylate Hydrate (4 nM; 24 hours) results in a large shift in cell-cycle populations from G1 and G2-M to S-phase with an accompanied induction of H2AX … WebFeb 8, 2024 · RPA is a crucial single strand DNA-binding protein complex that orchestrates DNA replication and repair 22, 23, 24. Under conditions of replication stress, the RPA2 … hjjk harjoitusajat