Myc-tagged full-length human Rootletin was a gift from Erich Nigg (Addgene plasmid # 41167)24. In a previous study, R1, R123, and R234-deletion constructs were generated from mouse Rootletin and GFP-tagged35; these constructs were thoroughly humanized, and the GFP tag was replaced with Myc or … See more A schematic of our custom-made CNOM16 is shown in Fig. S2a. Owing to recent advances in supercontinuum (SC) generation technology, our CNOM system has extended to ultra-broadband spectroscopic live cell … See more Because the excitation wavelength of the original custom-made penta-modal CNOM was ≥1064 nm, we could not excite typical fluorophores such as Alexa Fluor 488. In order to achieve simultaneous measurement of SHG … See more Antibodies were obtained from the indicated suppliers: goat anti-Rootletin for immunostaining (sc-67824) and mouse anti-Rootletin for immunoblotting (sc-374056; Santa Cruz … See more COS7 and U2OS cells (ATCC, Manassas, VA) were cultured in DMEM containing 10% fetal bovine serum. They were authenticated by species-specific PCR49 or by short tandem repeat (STR) profiling techniques, … See more Web10 Jun 2024 · Staining of unfixed frozen retinal sections was performed at 3 weeks following treatment at 1-2 months of age. (FIG. 2B) Fluorescence images of both forms of …
Retinitis: Types, Symptoms, Causes, Treatment - WebMD
WebThe striated ciliary rootlet is a prominent cytoskeleton originating from basal bodies of ciliated cells. Although a familiar structure in cell biology, its function has remained … Web18 Jan 2024 · retinal tissues using ARL2BP and GAPDH (loading control) directed antibodies. Protein molecular weight in kilodaltons (kDa) is indicated on the left. ... (D, E) … pheromones roll on
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WebRetinitis is a disease that threatens vision by damaging the retina -- the light-sensing tissue at the back of your eye.Although there's no cure, there are steps you can take to protect … WebPurpose: : To identify in mouse retina potential interacting partners of 7R, a novel retinal protein characterized as a Golgi-associated protein in stably transfected HEK 293 cells. ... Web13 May 2024 · Complete neuron wiring can been reconstructed, and whole eukaryotic cells imaged, at less greater 10 nms isotropical resolution. pheromones production