WebPure DNA has an A 260 /A 280 ratio of 1.8–2.0 in 10 mM Tris·Cl, pH 8.5. ... PicoGreen is a highly sensitive measure of dsDNA and can measure as little as 20 pg dsDNA in a 200 µl assay volume. Indeed, DNA concentrations from 500 pg/ml to 500 ng/ml can all be measures using a single dye concentration. WebN2 - We describe the development of a novel, robust assay system for determining the changes in activity of proprotein converting enzymes. An assay for prolyl oligopeptidase (POP) activity was constructed using a peptide-streptavidin substrate coupled to magnetic microspheres and cleavage was detected by loss of streptavidin on the MAGPIX reader.
Overview of Protein Assays Methods - Thermo Fisher Scientific
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Guide to Enzyme Unit Definitions and Assay Design
WebThe assay is highly selective for small RNA over rRNA or large messenger RNA (mRNA) (>1,000 bp). Small RNA in pure samples has reproducibly been quantified at levels as low as 0.5 ng in the assay tube. The assay detects all types of small RNA, including microRNA and short interfering RNA (siRNA), both single- and double-stranded. WebTo overcome the cost disadvantages of the Oncotype DX assay and the turnaround time, a multigene assay was examined to compare the correlation of the RS and the predicted score (PS) of the present study. Paraffin-embedded tissues of 50 cases with early-stage estrogen receptor (ER)-positive breast cancer, who underwent the Oncotype DX test were ... WebIn the ABTS assay, also known as Trolox equivalent antioxidant capacity (TEAC) assay, the green–blue stable radical cationic chromophore, 2,2-azinobis- (3-ethylbenzothiazoline-6-sulfonate) (ABTS•+) is produced by oxidation, and has absorption maxima at 414, 645, 734, and 815 nm ( Prior et al., 2005 ). In the original assay, metmyoglobin was ... dialysis technician jobs in nj