WebpULK1(S757)(CS6888),rabbitanti-mTOR(CS2972),andrab-bit anti-phospho-mTOR (S2448) (CS2971S) from Cell Signal-ingTechnologyandmouseanti- -actin(sc-47778)fromSanta Cruz Biotechnology. Secondary antibodies were donkey anti-rabbit IRDye 680RD and donkey anti-mouse IRDye 800CW from LiCor. Blots were stripped between incubations using WebpULK1-S555. Data are shown as mean phosphorylation (arbitrary units) ± s.e.m.; n = 3. Statistical significance vs. vehicle-treated WT was calculated by one-way ANOVA with Dunnett’s multiple-comparisons test. Representative immunoblots are shown. C) HEK293T cells expressing FLAG-tagged a1b1g1 or a2b1g1 were treated with 250 nM rapamycin or
Lipocalin-2 inhibits autophagy and induces insulin resistance in …
WebJul 15, 2016 · First, Lcn2 reduced pULK1 S555, increased pULK1 S757 and reduced LC3-II levels determined by Western blotting. We validated the use of DQ-BSA to assess … WebAug 28, 2024 · The densities of p-ATG13, p-BECN1, and p-ULK1 S757 are quantified and showed in the right panel. f HeLa cells were treated with different concentrations of PKC … raspored sati za printanje
An AMPKα2-specific phospho-switch controls lysosomal targeting …
WebFeb 13, 2024 · Primary antibodies against Met, pMet (Y1234/1235), p62, LC3, mTOR, pmTOR (S2448), pULK1 (S757), Atg5, Beclin-1, NBR1, and secondary horseradish peroxidase-conjugated goat anti-rabbit and anti ... WebJan 24, 2024 · Besides, pAMPK T172 and pULK1 S556 were decreased, and pmTOR S2448 and pULK1 S757 were increased. Cell experiments showed that the level of … WebDec 6, 2024 · Reactivation of mTORC1 in full medium within 1 h was observed by phosphorylation of downstream substrates pULK1 (S757), pWIPI2 (S413), and pAMBRA1 (S52), ... Interestingly, although we observed reduced ULK1 S757 phosphorylation in USP32 KO cells and also accumulation of nuclear TFEB as a consequence of reduced mTORC1 … raspored sjedala u autobusu