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Pulk1 s757

WebpULK1(S757)(CS6888),rabbitanti-mTOR(CS2972),andrab-bit anti-phospho-mTOR (S2448) (CS2971S) from Cell Signal-ingTechnologyandmouseanti- -actin(sc-47778)fromSanta Cruz Biotechnology. Secondary antibodies were donkey anti-rabbit IRDye 680RD and donkey anti-mouse IRDye 800CW from LiCor. Blots were stripped between incubations using WebpULK1-S555. Data are shown as mean phosphorylation (arbitrary units) ± s.e.m.; n = 3. Statistical significance vs. vehicle-treated WT was calculated by one-way ANOVA with Dunnett’s multiple-comparisons test. Representative immunoblots are shown. C) HEK293T cells expressing FLAG-tagged a1b1g1 or a2b1g1 were treated with 250 nM rapamycin or

Lipocalin-2 inhibits autophagy and induces insulin resistance in …

WebJul 15, 2016 · First, Lcn2 reduced pULK1 S555, increased pULK1 S757 and reduced LC3-II levels determined by Western blotting. We validated the use of DQ-BSA to assess … WebAug 28, 2024 · The densities of p-ATG13, p-BECN1, and p-ULK1 S757 are quantified and showed in the right panel. f HeLa cells were treated with different concentrations of PKC … raspored sati za printanje https://baileylicensing.com

An AMPKα2-specific phospho-switch controls lysosomal targeting …

WebFeb 13, 2024 · Primary antibodies against Met, pMet (Y1234/1235), p62, LC3, mTOR, pmTOR (S2448), pULK1 (S757), Atg5, Beclin-1, NBR1, and secondary horseradish peroxidase-conjugated goat anti-rabbit and anti ... WebJan 24, 2024 · Besides, pAMPK T172 and pULK1 S556 were decreased, and pmTOR S2448 and pULK1 S757 were increased. Cell experiments showed that the level of … WebDec 6, 2024 · Reactivation of mTORC1 in full medium within 1 h was observed by phosphorylation of downstream substrates pULK1 (S757), pWIPI2 (S413), and pAMBRA1 (S52), ... Interestingly, although we observed reduced ULK1 S757 phosphorylation in USP32 KO cells and also accumulation of nuclear TFEB as a consequence of reduced mTORC1 … raspored sjedala u autobusu

Targeting autophagy potentiates antitumor activity of Met-TKIs …

Category:SopB-mediated activation of mTORC1 inhibits autophagy

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Pulk1 s757

ULK1 induces autophagy by phosphorylating Beclin-1 …

WebFurthermore, we monitored increased levels of pp70S6K (T389), pULK1 (S757), and pAKT (S473), suggesting increased mTOR signaling ( Figure 5K, Figure S5U, S5V). ... View in full-text Context 19 WebMay 21, 2024 · First, Lcn2 reduced pULK1 S555, increased pULK1 S757 and reduced LC3-II levels determined by Western blotting. We validated the use of DQ-BSA to assess autolysosomal protein degradation and this together with MagicRed cathepsin B assay indicated that Lcn2 reduced lysosomal degradative activity.

Pulk1 s757

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WebUlk1 is phosphorylated at S757, while mTOR kinase activity is partially suppressed under hyperosmotic stress. (A) Wild-type MEFs and T24 cells were cultured with amino acid … WebFeb 1, 2024 · Western blot analysis exhibits the effect of Alda-1 on the phosphorylation of ULK1 with high glucose exposure. G: Representative gel blots of pULK1and β-actin …

WebNational Center for Biotechnology Information WebJan 18, 2024 · Whole cell extracts from MEFs, ESCs, and iPSCs were harvested for western blot using anti-p-ULK1(S555), anti-p-ULK1(S757), and anti-ULK1 antibodies. ULK1 …

WebMay 19, 2013 · Whole cell lysates were immunoblotted with pULK1 S757 (mTORC1-target site), pS6K and ULK1 antibodies (bottom panels). Two unique experiments were performed. (c) Wild-type or FIP200 -/- MEF were incubated under nutrient rich, amino acid deprived or Torin-1 (+T, an mTOR inhibitor) conditions. WebNov 12, 2024 · PVT elevates ULK1 mediated autophagy in PDAC cells. Western blot analysis of pULK1(S757), ULK1, Atg101, FIP200, Beclin-1, Atg5, and LC3A/B in (A) PO2 (B) BxPC3 (C) L3.6pl, (D) AsPC1, and (F ...

WebNational Center for Biotechnology Information

WebEtOH altered ULK1 S555 and S757 phosphorylation in a time- and AMPK-dependent manner. The activation of AMPK and ULK1 was associated with increased BECN1 (S93, … dr raajit chananaWebWestern blot analysis of extracts from various cell lines using Phospho-ULK1 (Ser757) (D7O6U) Rabbit mAb. Confocal immunofluorescent analysis of MCF7 cells, untreated … raspored sjednica vlade rhWebAll lanes : Anti-ULK1 (phospho S757) antibody [EPR22265-9] (ab229909) at 1/1000 dilution Lane 1 : MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate Lane 2 : … raspored siječanj 2023WebMay 19, 2013 · Whole-cell lysates were immunoblotted with pULK1 S757 (mTORC1-target site), pS6K and ULK1 antibodies (bottom panels). Two unique experiments were performed. raspored slaganjaWebEtOH altered ULK1 S555 and S757 phosphorylation in a time- and AMPK-dependent manner. The activation of AMPK and ULK1 was associated with increased BECN1 (S93, S14) and PIK3C3/VPS34 (S164) phosphorylation as well … raspored sjedenja na vjenčanju downloadWebFeb 1, 2024 · Western blot analysis exhibits the effect of Alda-1 on the phosphorylation of ULK1 with high glucose exposure. G: Representative gel blots of pULK1and β-actin (used as loading control) with specific antibodies; H:ULK1-to-β-actin;I: pULK1-s757-to β-actin;J: pULK1-s555-to β-actin;K: pULK1-s317-toβ-actin.All proteins were normalized to β-actin. raspored sjednica vstvraspored sjednica