Psin-ef2 vector
WebNov 17, 2024 · GFP-human Med15 and TagRFP-human DYRK3 fusion protein were subcloned into the lentiviral expression vector pSin-EF2 by ClonExpress II One Step Cloning Kit (Vazyme Biotech, C112). Primers used to clone lentiviral vectors are provided in Additional file 6: Table S3. The human embryonic kidney 293T cell line was used as a host … WebFull Sequences from Depositor (1) Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between …
Psin-ef2 vector
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WebSep 14, 2024 · The empty vector pSin-EF2-Pur was used as a control. The plasmids were verified by sequencing. For stable knockdown of ELMO1, GV248-puro [Shanghai Genechem Co., Shanghai, China] containing hairpin sequence targeting ELMO1 or a control plasmid [control hairpin] was co-transfected with psPAX2 and pMD2G [Addgene, Cambridge, MA, … WebpSin (Search Vector Database) Vector type Synthetic Biology Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) NEB Stable Copy number Low Copy Gene/Insert Gene/Insert name Sindbis Glycoproteins Cloning Information Cloning method Unknown Resource Information Supplemental Documents
WebJan 22, 2015 · The pri-miR-34c sequence was cloned into the lentiviral plasmid pSin-EF2-puromycin (Addgene, Cambridge, MA, USA); pSin-EF2-miR-34c or negative control empty pSin-EF2 vector was then co-transfected ... WebApr 6, 2024 · Briefly, the pSin-EF2-TIPE3-HA or empty vector, as well as the lentivirus packaging plasmids psPAX2 and pMD2.G, were co-transfected into 293FT cells using the calcium phosphate method. After...
WebPsin Ef2 Puromycin Lentiviral Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article … http://www.labbase.net/Supply/SupplyItems-4229347.html
WebMay 11, 2024 · abstract type AbstractPolynomial end """ Polynomial <: AbstractPolynomial Polynomials written in the canonical form --- Polynomial(v::T) where T<:Union{Vector{<:Real}, NTuple{<:Any, <:Real}}) Construct a `Polynomial` from the list of the coefficients. The coefficients are assumed to go from power 0 in the ascending order.
WebJan 8, 2024 · The pSin-EF2-puro-ARNTL, pSin-EF2-puro-vector and psin-EF2-puro-CDK5 plasmids were obtained from Land. Hua Gene Biosciences (Guangzhou, China), and the psin-EF2-puro-vector plasmid served as a control. To generate stable cell lines, lentivirus packing expression plasmids were co-transfected into 293 T cells for 48 h. Then, SUNE1 … tim hortons nhl trading cards checklistWebOct 24, 2012 · These transgene-carrying iPSCs (pSin-EF2-gene-Pur vector system) underwent massive cell death (data not shown) after differentiation toward MSC lineages using a reported protocol established for deriving MSCs from hESCs . We used the same protocol to guide hESCs (H1 and H9) and were successful in generating MSC-like cells … parkinson white diseaseWebJul 18, 2024 · The full length of CTBP1-DT was generated by subcloning the PCR-amplified human CTBP1-DT sequence and cloned into pSin-EF2-vector. The DDUP ORF with FLAG-tag expression plasmid was generated by cloning the full length of CTBP1-DT. tim hortons northampton menuWebFeb 3, 2024 · The AKR1B10 CDS (coding DNA sequence) was cloned into the lentiviral expression vector pSIN-EF2-Puro (hereinafter referred to as pSIN-EF2) to construct the lentiviral vector pSIN-EF2/AKR1B10. The method to establish stable AKR1B10-expression cell line has been described previously 23. AKR1B10-shRNA stable transfection using … tim hortons northampton opening hoursWebSystemic delivery of pSin-EF2-ELMO1-Pur facilitates continuous ELMO1 expression in colonic tissues. [A] Immunohistochemical staining of ELMO1 in the colons of untreated mice or mice treated with... tim hortons nordel wayWebJan 7, 2024 · Product Name : pSin-EF2-Sox2-Pur article : bacterial resistance : Ampicillin cloning : growth notes : The psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector. origin : 37 pi : plasmid copy : SINF-EF-G (from Dr. Robert Hawley, George Washington University) was modified to make the lentiviral backbone. parkinson what is itWebJan 22, 2015 · The pri-miR-34c sequence was cloned into the lentiviral plasmid pSin-EF2-puromycin (Addgene, Cambridge, MA, USA); pSin-EF2-miR-34c or negative control empty … parkinson who