Mouse beta actin real-time pcr primers
NettetPrimePCR™ Template for SYBR® Green Assay: BETA-ACTIN, Pig. Reaction: 4 billion copies/200 µl desalted. Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay. Nettet24. jul. 2024 · Quantitative reverse transcription PCR (RT-qPCR) is one of the most efficient, reliable and widely used techniques to quantify gene expression. In this study, we evaluated the performance of six ...
Mouse beta actin real-time pcr primers
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NettetDownload Table Real-time PCR primer sequences for gene targets in mice. from publication: Increased Renal Methylglyoxal Formation with Down-Regulation of PGC-1α … NettetDesign and Validation of Real-Time PCR Primers-test. Sensitivity. Accurate detection of 20 copies. Specificity. Amplicon sequence validated with next generation sequencing …
NettetReal-time PCRs were performed in a final volume of 20 μl containing 0.5 μM of forward and reverse primers (Integrated DNA Technologies, Coralville, Iowa, USA), 10 μl of Light Cycler 480 SYBR ... Nettet27. apr. 2024 · We developed a set of carefully designed primer pairs and PCR conditions to selectively determine the expression of mouse, human or rat α-SMA and ß-actin …
Nettet27. apr. 2024 · Applying our mouse α-SMA specific primer pairs in real-time PCRs containing the non-specific ß-, γ-cyto- or γ-smooth-actin DNA templates (Fig. 2), or our mouse ß-actin specific primer pair in real-time PCRs containing α-SMA, or γ-smooth actin specific DNA templates, PCR products were not observed (Fig. 3). NettetDownload Table Real-time PCR primer sequences for gene targets in mice. from publication: Increased Renal Methylglyoxal Formation with Down-Regulation of PGC-1α-FBPase Pathway in Cystathionine ...
NettetBeta-actin--an unsuitable internal control for RT-PCR Despite reports confirming cell-cycle dependent gene expression and a number of studies describing specific circumstances in which beta-actin is also regulated, the mRNA for beta-actin remains a widely used housekeeping gene internal control.
Nettet14. apr. 2024 · RT-PCR and real-time qPCR revealed that the mRNA expression of ovarian function markers ... 10 pmol/µL forward and reverse primers and 200 ng of … estate sales in farmington ctNettet27. apr. 2024 · Results: Real-time PCRs using self-designed mouse, human and rat specific α-SMA or ß-actin primer pairs resulted in the specific amplification of the artificial DNA templates corresponding to mouse, human or rat α-SMA or ß-actin, however ß-actin showed cross-reaction with the housekeeping γ-cyto-actin. fire box home depotNettetThe Applied Biosystems™ Mouse ACTB (actin, beta) Endogenous Control (VIC™ ⁄ MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene … firebox humble tx natural gas grillsNettetBuy Actb, Mouse actin, beta, Real Time PCR Primer Set, item number: VMPS-96 from RealTimePrimers at Biomol! Primers are provided as a 40 µl solution containing both … estate sales in frankenmuth michiganNettet2. feb. 2024 · In experimental research using mice, 18S rRNA, glyceraldehyde-3-phosphate dehydrogenase (Gapdh) or beta-actin (Actb) 7 are most frequently used as … estate sales in farmington nmNettet8. okt. 2012 · Background One requisite of quantitative reverse transcription PCR (qRT-PCR) is to normalise the data with an internal reference gene that is invariant regardless of treatment, such as virus infection. Several studies have found variability in the expression of commonly used housekeeping genes, such as beta-actin (ACTB) and … firebox houseNettetSupplementary Table I. DNA sequences of the primers used for RT-PCR. Mouse cDNAs RT-PCR ... GenBank accession No. β-actin Real-time & Traditional Forward, 5 ´-ATCCGTAAAGACCTCTATGC-3´ Reverse, 5´-AACGCAGCTCAGTAACAGTC-3´ NM_007393 Ccl2 Real-time Forward, 5´-TCAGCCAGATGCAGTTAACG-3´ Reverse, … estate sales in farmington hills mi