NettetThe plot of substrate concentration against the rate of reaction resembles a rectangular hyperbola. To visualize Michaelis-Menten kinetics practically, Lineweaver-Burk plot is the easiest way to do it as it plots the inverse of the reaction rate (1/r) against the inverse of substrate concentration (1/ [S]). Nettet1. sep. 2024 · As shown in Figure 13.14, when we display kinetic data using as a Lineweaver-Burk plot it is easy to determine which mechanism is in effect. For example, an increase in slope, a decrease in the x -intercept, and no change in the y -intercept indicates competitive inhibition.
Michaelis-Menten vs. Lineweaver-Burk Plots - Clutch Prep
NettetHanes–Woolf plot. In biochemistry, a Hanes–Woolf plot, Hanes plot, or plot of against , is a graphical representation of enzyme kinetics in which the ratio of the initial substrate concentration to the reaction velocity is plotted against . It is based on the rearrangement of the Michaelis–Menten equation shown below: Nettet24. sep. 2016 · How to Calculate Km and Vmax using Lineweaver Burk Plot Dr.Mungli 64.6K subscribers 81K views 6 years ago In this video I have explained how to calculate Km and Vmax of an … facts of animal testing
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NettetLineweaver-Burk plot for an enzyme displaying sequential enzyme kinetics. Secondary plots of the slope and intercept (Figure 4) of the data depicted in Figure 3provide a means to determine the Vmax(both [A] and [B] saturating) and other kinetic constants (Ka, Kb, Kia) describing sequential enzyme kinetics. Figure 4. NettetLineweaver-Burk plot and Eadie-Hofstae methods; Competitive and non-competitive. 3 inhibition. Chapter 8. ... and an innovative vertical format for explaining reaction mechanisms, Fundamentals takes a modern approach: primary organization is by functional group, beginning with the simple (alkanes) ... Nettet23. aug. 2024 · Lineweaver-Burke (the "double reciprocal" plot) The Michaelis-Menten equation can be rearranged by taking the reciprocal, to yield: If X = 1/[S] and Y=1/ V … facts of anne frank