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WebApr 3, 2011 · Synonym Discussion of Include. to take in or comprise as a part of a whole or group; to contain between or within; to shut up : enclose… See the full definition WebAs a general rule of thumb, the excluded volume (Vo) is approximately equal to one third of the column volume, the included volume is approximately equal to two thirds of the column volume In gel filtration the resolution is a function of column length (the longer the better) b daht unc