WebMar 23, 2024 · The PCR products from the cDNA of the GATA2 gene were purified by cutting the band of the expected size from 1.5% agarose gels and eluting it with the FastGene Gel/PCR Extraction Kit (Nippon Genetics, Tokyo, Japan). WebCatalog number: K0691. Thermo Scientific GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE …

A novel germline GATA2 frameshift mutation with a premature

WebJul 15, 2024 · PCR was conducted using KOD One PCR Master Mix (TOYOBO) as follows: 40 cycles of 98 °C for 10 s, 45 °C for 5 s, and 68 °C for 5 s. Each PCR product was purified using the FastGene Gel/PCR Extraction Kit, cloned into pGEM-T easy vector using competent quick DH5α E. coli , and sequenced by Eurofins Genomics. WebFastGene Gel/PCR Extraction Kit is a bind-wash-elute spin column-based kit. Amplified DNA fragments can be extracted and purified from agarose gel slices or PCR samples. … geoffrey bache smith poetry

Patch cloning method for multiple site-directed and saturation ...

WebDNA fragments purified with FastGene Gel/PCR Extraction Kits are ready for direct use in all common downstream applications, like sequencing, ligation and transformation, … WebJan 18, 2024 · The barcoded PCR products were mixed in equal amounts and then purified using FastGene Gel/PCR Extraction Kit (Nippon Genetics, Germany). The volume of the mixed and purified PCR products was adjusted to 20 to 30 ng/μL. The library was prepared using Ligation Sequencing 1D kit (SQK-LSK108_109; ONT, Oxford, UK) and NEBNext … WebNov 30, 2024 · PCR products were extracted and cleaned with a FastGene Gel/PCR Extraction Kit (NIPPON Genetics). Amplicon sequencing libraries of cleaned PCR products were prepared using a KAPA Hyper Prep Kit (NIPPON Genetics Co., Ltd.) without fragmentation. Libraries were multiplexed and 300-bp paired-end reads were sequenced … chris macintosh scam