WebAug 23, 2024 · To mitigate this, we also developed a counterscreen assay that identifies potential interferers at the levels of both the luciferase enzyme reporter and the mechanism responsible for a glow-like response. Interestingly, we also found that the peptide substrate for chymotrypsin-like proteasome activity was not specific and was likely partially ... WebAug 28, 2009 · The purpose of this assay is to determine the cytotoxicity of compounds identified as STAT3 inhibitor probe candidates. This assay also serves as a counterscreen to identify false positives and non-selective …
Probe Development Efforts to Identify Novel …
WebSep 1, 2013 · The counterscreen protocol selected for HTS is identical to the primary HTS assay protocol with the exception that the hV2R[L 83 Q] cells are incubated in the … WebOct 12, 2010 · We pursued a Center-based Initiative with transfection studies testing the ability of various chemical scaffolds to inhibit LRH-1-mediated activation of the Cyp19-Aromatase-luciferase and the StAR … highly dependable definition
Identification of Potent and Selective
WebFeb 1, 2024 · This chapter describes (a) the basic principles of proximity assays, (b) common sources of compound-mediated assay interference in homogeneous proximity assays, and (c) counter-screens and other … WebMay 29, 2024 · Firefly luciferase is an enzyme that has found ubiquitous use in biological assays in high-throughput screening (HTS) campaigns. The inhibition of luciferase in … WebThe assays used for HTS of hit compounds are called primary screen. Once hits have been confirmed in the primary screen, assays need to be designed to eliminate false positives caused by the effect of compounds on the assay technology or format. Counter-screen … highly dense acoustic foam