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Contigs and singletons

WebThe full-length enriched EST data were clustered and assembled into unique sequences, contigs, and singletons. The PiGenome provides functional annotation, identification of … http://scorreia.com/research/singletons.pdf

Transcriptome sequencing in an ecologically important tree …

WebJan 23, 2014 · Contigs and singletons had an average GC content of 36% and 35%, respectively . This was lower than the GC content (46%) of the red flour beetles ( Tribolium castaneum ) transcriptome suggesting that the genome of L. decemlineata could be very rich in AT as shown in other insects [40] . WebIn each collection, 16% of the contigs/singletons have no matches in databases and 3-5% encode hypothetical proteins; novel hypothetical proteins (not found within the female … how to create an elearning platform https://baileylicensing.com

(PDF) Rapid transcriptome characterization for a nonmodel …

WebNov 20, 2009 · The number of contigs and singletons is much higher compared to the ZM_BF assembly because the proportion of mate-pairs was much lower, the ESTs are from different cultivars, and the quality of some sets of ESTs may be low. There were 10,618 contigs containing only ESTs from the ZM_BF libraries, representing 10k potential … WebJul 25, 2013 · Results. Total RNA was isolated from the mantle, gill, liver, siphon, gonad and muscular foot tissues. High-throughput deep sequencing of S. constricta using 454 … WebJul 26, 2011 · Our reads were assembled into 93,627 contigs (38,256,084 bp) and 309,074 singletons (69,524,702 bp), with an overall total of 402,701 unigenes (107,780,786 bp) that were deposited in a newly developed database called SiESTa (Silene EST annotations) (Table 1). All reads may be accessed under the accession number ERP000371 in the … microsoft power supply 102w

High-Throughput Sequence Analysis of Turbot - PLOS

Category:转录组高通量测序中，reads、contigs、scaffold …

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Contigs and singletons

Summary of EST data constructed from Closterium ehrenbergii . Contigs …

WebSingleton read; and; Discordant mate pairs; ... Contigs are long continuous stretch of sequence results from overlapping short reads. When you do a paired-end sequence, … Webof which 𝐺𝑆are singletons. These need to be considered—together with𝐾—as estimated parameters,soourpreviousequationbecomes: 𝑞∗ = (𝑀 + 𝑀𝑆 𝑀 + 𝑀 𝑆− 1)(𝑁 + 𝑀𝑆− 1 𝑁 + 𝑀− 𝐾 − 𝐺) (3) In this case, dropping singleton groups not only removes 𝑀𝑆from the equation—

Contigs and singletons

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WebAug 14, 2024 · The genes on the contigs that were a mixture of singletons and non-singletons tended to emerge from the same species or genus. Only 8,557 (0.8%) of all mixture contigs in the oral microbiome contained potential cross-genus HGT. In the gut, there were 33,224 of these cross-genus, mixture contigs, a total of 1.8%. WebDownload Table BLASTX hits of contigs and singletons in the eight individual libraries with different proteomes from publication: Comparative high-throughput transcriptome sequencing and ...

Web1 Dr. Julia Weiss Genetics Institute of Plant ... - Plant Physiology WebEnter the email address you signed up with and we'll email you a reset link.

WebSep 26, 2010 · After removing redundancy, the resulting consensus sequences of contigs and singletons were again mined for GSS-SSRs for further analysis. Marker development and polymorphism detection. Primer3 software (Rozen and Skaletsky 2000) was used to design PCR primers flanking SSR sequences from the non-redundant SSR-containing … Webof which 𝐺𝑆are singletons. These need to be considered—together with𝐾—as estimated parameters,soourpreviousequationbecomes: 𝑞∗ = (𝑀 + 𝑀𝑆 𝑀 + 𝑀 𝑆− 1)(𝑁 + 𝑀𝑆− 1 𝑁 + 𝑀− 𝐾 − 𝐺) (3) In this …

WebJun 18, 2015 · Over half of these sequences, 3,161 contigs and 9,389 singletons, retrieved matches from at least one related grass proteome, indicating ‘conserved’ genes among grasses . Among these, 2,555 ...

WebMar 16, 2010 · A combination of reference-based and de novo assemblies yielded 63,657 contigs, with 239,793 reads remaining as singletons. Based on sequence similarity with known proteins, these sequences represent approximately 17,000 unique genes, many of which are well covered by contig sequences. microsoft power supply 127 watt us7-00001WebAug 6, 2012 · BLAST searches for all contigs and singletons obtained from M. amblycephala were performed against the nr protein database using the BLASTx algorithm and an arbitrary expectation value of E −10 . Among a total 100,477 unigenes (contigs and singletons), 40,687 (40.5%) showed at least one significant alignment to an existing … microsoft power supply model 1706WebNational Center for Biotechnology Information how to create an electronic document